NOTEBOOK ON STRUCTURAL BIOLOGY TECHNIQUES

This book describes the basic techniques required in learning structural biology. Apart from the classical and standard approaches like X-ray crystallography and SAXS (small angle X-ray scattering), it has quite an extensive chapter on the most recent approach; namely, cryo Electron Microscopy.

The material is partly pedagogical, allowing readers to learn the subject, including some advanced topics. The application of these techniques to a specific example (Ribonucleotide Reductase) is discussed. At the end there is a detailed Mathematical Supplement, broken into six sections, that develop (from basic principles) the various mathematical machinery needed to learn the techniques of structural biology. It elaborates on the key mathematical concepts mentioned in the various chapters.

Additionally, there is a collection of Ipsita's papers highlighting her seminal contributions, especially in the data processing of cryo EM.

Contents:

• Preface
• X-ray Crystallography
• Basics of Electron and Cryo-Electron Microscopy
• Single Particle Cryo-Electron Microscopy
• Small Angle X-Ray Scattering
• Ribonucleotide Reductases (RNRs)
• Solution Structure of the dATP-Inactivated Class I Ribonucleotide Reductase From Leeuwenhoekiella blandensis by SAXS and Cryo-Electron Microscopy
• Nucleotide Binding to the ATP-cone in Anaerobic Ribonucleotide Reductases Allosterically Regulates Activity by Modulating Substrate Binding
• Mathematical Supplement/Appendix
• Ipsita: About Her

Readership: Persons in academia related to the subjects of Physics, Chemistry and Biology would find it useful. Can be used at both undergraduate and graduate levels. Interdisciplinary courses will find the book particularly suitable. For example, a course in physics or chemistry designed to include modern biology will find the book appealing.